
Fabian David Nonis
Self-motivated professional with a broad background in cellular/molecular biology and biochemistry technologies. Demonstrated success with multiple project management... | United States
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Fabian David Nonis’s Emails fa****@ce****.org
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Fabian David Nonis’s Location United States
Fabian David Nonis’s Expertise Self-motivated professional with a broad background in cellular/molecular biology and biochemistry technologies. Demonstrated success with multiple project management and organization in the laboratory. Able to set priorities and implement decisions to meet deadlines. Outstanding critical thinking skills demonstrated by managing successfully research projects. Key Areas of Expertise Cloning – vector/plasmid construction, vectors-insertion, ligation, expression, site directed mutagenesis – Genome editing, CRSPR Cas9 - plasmid purification - DNA sequencing and analysis – restriction enzyme analysis – design of DNA oligonucleotide - mammalian cell stable and transient transfection – mammalian cell culture (primary and immortalized) – Cell Banking – IPSC/Stem cells – IPSC differentiation - Sterile/aseptic techniques – culture of microorganisms (bacteria and yeast) – Inventory of microbial strains – lentivirus production and transduction - recombinant protein engineering, expression, purification and analysis (ELISA, SDS-PAGE) - protein interaction (yeast-two-hybrid system, Co-immunoprecipitation, GST pull down, fluorescence microscopy) – immunocytochemistry – immunoassays – luciferase assay - flow cytometry (FACS analysis) - DNA/RNA isolation - PCR, RT-PCR – biostatistics – data analysis – rat and mouse handling – mouse knock out models. Gene editing CMO projects managing, organization and execution. GMP and clean room experience and management. - proficient in English and Spanish languages.
Fabian David Nonis’s Current Industry Viacyte
Fabian
David Nonis’s Prior Industry
Universidad Nacional De Cordoba
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Goethe University
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Uc San Diego
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Consulting
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Cedars Sinai
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Own Bussiness
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Tanabe Research Laboratories Usa
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Viacyte
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Work Experience

Viacyte
Supervisor/Lead Senior Scientist in Cellular and Molecular Biology, focused on Gene Editing Projects
Fri Jan 01 2021 00:00:00 GMT+0000 (Coordinated Universal Time) — Present
Tanabe Research Laboratories Usa
Supervisor/Lead Scientist in cellular and molecular biology
Wed Jan 01 2020 00:00:00 GMT+0000 (Coordinated Universal Time) — Fri Jan 01 2021 00:00:00 GMT+0000 (Coordinated Universal Time)
Own Bussiness
Molecular Biology Scientist/Consultant
Mon Jan 01 2018 00:00:00 GMT+0000 (Coordinated Universal Time) — Tue Jan 01 2019 00:00:00 GMT+0000 (Coordinated Universal Time)
Cedars Sinai
Postoctoral Scientist
Thu Jan 01 2015 00:00:00 GMT+0000 (Coordinated Universal Time) — Sun Jan 01 2017 00:00:00 GMT+0000 (Coordinated Universal Time)
Consulting
Cunsulting/teaching
Tue Jan 01 2013 00:00:00 GMT+0000 (Coordinated Universal Time) — Wed Jan 01 2014 00:00:00 GMT+0000 (Coordinated Universal Time)
Uc San Diego
Postdoc Fellow
Thu Jan 01 2009 00:00:00 GMT+0000 (Coordinated Universal Time) — Sun Jan 01 2012 00:00:00 GMT+0000 (Coordinated Universal Time)
Goethe University
PhD student
Sat May 01 2004 00:00:00 GMT+0000 (Coordinated Universal Time) — Wed Oct 01 2008 00:00:00 GMT+0000 (Coordinated Universal Time)
Universidad Nacional De Cordoba
Undergraduate student trainee
Tue Jan 01 2002 00:00:00 GMT+0000 (Coordinated Universal Time) — Wed Jan 01 2003 00:00:00 GMT+0000 (Coordinated Universal Time)